thebestgene.com (Bestgene Inc) provides fast, cheap and high quality Drosophila transgenic (transgene/injection/microinjection/p-element transformation) service. (Drosophila, fly, fruit fly, P-element, embryo, Transgenic, microinjectoin, injection, transformation, transgene, service, facility, research, transgene service, Drosophila transgene, fly transgene, fruit fly transgene, Drosophila injection, fly injection, fruit fly injection, Drosophila microinjection, fly microinjection, fruit fly microinjection, p-element transformation service, Drosophila embryo microinjection, embryo microinjection, transposon injection, P-element injection, transgenic fruitfly, transgenic fly, transgenic Drosophila)
- The cheapest price for the best service! Starting from $200 per injection
- User friendly system! Real-time tracking of service progress online
- Inject 200+ embryos per service
- Deliver transformed and/or balanced flies. We do the crosses!
- One shipping & handling charge per order! No matter how many services purchased
- Selection of w1118, yw or your own strain for transposable-element injection
- PhiC31 integrase-mediated site-specific transgenesis - the broadest selection of attP sites
- Screen for w+, y+, v+ and/or GFP/RFP/DsRed/YFP
- MiMIC injection service
- CRISPR, TALEN and ZFN injection service
- Over 50,000 individual constructs were successfully injected and over 265,000 transformants delivered!
Drosophila Embryo Injection Services
We provide high quality Drosophila transgenic service to both research institutions and
companies. We offer you partial to full service ranging from DNA preparation, embryo
microinjection, screening for white+, yellow+, vermilion+ and/or Fluorescence (GFP/RFP/DsRed/YFP) phenotypes, to balancing crosses. Most importantly, the costs of our Drosophila injection services are more reasonable and affordable compared to that of others. With a large number of facilities and our highly experienced staffs, we are able to handle library-scale injection projects. Our friendly web-based database allows you to track your fly injection status, service history and more. See here for the sample page.
The Drosophila P-element transformation usually involves the injection of transposon-based construct
with the white and/or the yellow marker to w1118 or yw host line. We perform injections with your (or our) purified plasmid construct (helper P-element plasmid will be supplied by us unless you prefer to use your own)
and return you the injected lavae, or the stable lines or the balanced lines depending on your chosen service.
For details, please refer to the service page.
PhiC31 integrase-mediated transgenesis systems - P[acman] and FlyC31
PhiC31 integrase-mediated transgenesis systems are based on the site-specific bacteriophage PhiC31
integrase which mediates sequence-directed, irreversible and highly efficient integration between a
bacterial attachment site (attB) and a phage attachment site (attP).
We will inject your purified attB construct into your preferred PhiC31-containing attP
docking site embryos and return you the injected larvae, the stable lines without the
PhiC31 source or the balanced lines (for the FlyC31 system, you may also choose to remove most of the non-necessary sequences by the Cre-mediated excision, leaving ONLY the gene-of-interest flanking with one loxP and one attL site) depending on your chosen service. For details, please refer to the service page and the PhiC31 information page.
CRISPR, TALEN or ZFN Genome Editing
CRISPR/Cas9, TALEN, ZFN system are three powerful genome engineering tools that are composed of
programmable, sequence-specific DNA-binding modules associated with a nonspecific DNA nuclease,
which induce DBS (DNA double-strand breaks) that stimulate NHEJ (non-homologous end joining) or
HR (homologous recombination) repairing mechanisms at specific genomic locations. For details, please refer to the service page and the Genome Editing page.
Molecular Biology Services
We also provide related molecular lab services, e.g. DNA preparation (Qiagen Midiprep), BAC preparation (Qiagen Large Construct Maxiprep), PCR confirmation of recombination sites, orientation screening for MiMIC lines, RNA synthesis for CRISPR, TALEN and ZFN injection, etc. For details, please refer to the service page.